Active Recombinant Feline IL5 Protein, His-tagged

• Cat.No.: IL5-1048F
• Product Overview: Recombinant Feline IL5 (Accession # O77515) Ile20-Ser134, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Feline
• Tag: His
• Predicted N Terminal: Ile20
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.The ED50 for this effect is typically 2 - 8 ng/mL.
• Molecular Mass: Recombinant Feline IL5, His-tagged has a calculated MW of 14.6 kDa. In SDS-PAGE migrates as 20 kDa, reducing conditions.
• Protein length: Ile20-Ser134
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: IL5 interleukin 5 [ Felis catus (domestic cat) ]
• Official Symbol: IL5
• Synonyms: interleukin-5; interleukin-5; IL-5; T-cell replacing factor; TRF; eosinophil differentiation factor; interleukin 5 (colony-stimulating factor, eosinophil)
• Gene ID: 493803
• mRNA Refseq: NM_001009845.2
• Protein Refseq: NP_001009845.2
• UniProt ID: O77515
• Chromosome Location: chromosome: A1