Active Recombinant Human IL18RAP Protein, Fc Chimera

• Cat.No.: IL18RAP-1073H
• Product Overview: Recombinant Human IL18RAP (Accession # O95256), fused with , was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Phe20
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to inhibit the IL-18-induced response of KG-1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera.The ED50 for this effect is typically 2 - 6 µg/mL in the presence of 30 µg/mL of soluble IL-18 R alpha/Fc and 40 ng/mL of human IL-18.
• Molecular Mass: Recombinant Human IL18RAP, Fc Chimera has a calculated MW of 65.1 kDa (monomer). In SDS-PAGE migrates as 90-100 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: IL18RAP interleukin 18 receptor accessory protein [ Homo sapiens ]
• Official Symbol: IL18RAP
• Synonyms: IL18RAP; interleukin 18 receptor accessory protein; interleukin-18 receptor accessory protein; AcPL; CD218b; IL-1R7; IL-1R-7; IL-18RAcP; IL-1RAcPL; IL-18Rbeta; IL-18R-beta; IL-18 receptor beta; interleukin-1 receptor 7; interleukin-18 receptor beta; IL-18 receptor accessory protein; CD218 antigen-like family member B; ACPL; IL18RB; CDw218b; MGC120589; MGC120590
• Gene ID: 8807
• mRNA Refseq: NM_003853
• Protein Refseq: NP_003844
• MIM: 604509
• UniProt ID: O95256
• Chromosome Location: 2q12.1