Recombinant Human SNCG

• Cat.No.: SNCG-134H
• Product Overview: Recombinant Human γ-Synuclein produced inE.Coliis a single, non-glycosylated polypeptide chain. The γ-synuclein contains 127 amino acids and has a molecular mass of 13,300 Dalton. The γ-synuclein is purified by using conventional column chromatography techniques.

Specification

• Cat. No.: SNCG-134H
• Description: The γ-Synuclein (Originally known as a breast cancer specific gene product, BCSG1) is an acidic neuronal protein of 127 amino acids. The protein coding region of γ-synuclein was amplified by RT-PCR and cloned into an E.coli expression vector.
• Source: Escherichia Coli.
• Amino Acid Sequence: MDVFKKGFSI AKEGVVGAVE KTKQGVTEAA EKTKEGVMYV GAKTKENVVQ SVTSVAEKTK EQANAVSEAV VSSVNTVATK TVEEAENIAV TSGVVRKEDL RPSAPQQEGV ASKEKEEVAE EAQSGGD.
• Physical Appearance: Sterile filtered clorless solution.
• Formulation: The protein (1mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.1M NaCl and 1mM MgCl2.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Coomassie.
• Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of BMP-6 as a Reference Standard.
• Storage: Lyophilized BMP 6 although stable at room temperature for 3 weeks, should be stored desiccated below-18℃. Upon reconstitution Bone Morphogenetic Protein 6 should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: SNCG synuclein, gamma (breast cancer-specific protein 1) [ Homo sapiens ]
• Synonyms: SNCG; synuclein, gamma (breast cancer-specific protein 1); SR; BCSG1; synoretin; Gamma-synuclein; Persyn; Breast cancer-specific gene 1 protein; PERSYN; PRSN; Synoretin; OTTHUMP00000020013; synuclein, gamma (breast cancer-specific gene 1); breast cancer-specific protein 1
• Gene ID: 6623
• mRNA Refseq: NM_003087
• Protein Refseq: NP_003078
• MIM: 602998
• UniProt ID: O76070
• Chromosome Location: 10q23.2-q23.3
• Function: protein binding