||Recombinant HumanS100 Calcium Binding Protein A5 cloned from human cDNA was expressed inE.coli. The protein consists of the human S100A5 (residues 1-92, swissprotaccession P33763). MW = 21.5 kDa (dimer).
||Protein S100-A5 is a protein that in humans is encoded by the S100A5 gene.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain.
||> 90% by SDS-PAGE. The protein runs as a monomer giving a single band migrating at a molecular weight < 14 kDa.
||1mg/ml in MES 20mM, NaCl 100 mM, pH 6.5. The concentration is calculated from the absorbance at 280nm (ε280 = 4595 M-1 cm-1).
||Under the above described conditions, to avoid precipitation, the product can be concentrated to a maximum of 1.5mM (dimer).
||-20º C. The protein is stable 25 ºC for several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20º C. Avoid repeated freeze/defrost cycles.