||Recombinant Human S100 Calcium Binding Protein A9 cloned from human cDNA, was expressed inE.coli. The protein consists of the human S100A9 (residues 1-114) with a mutation at position 3: Cys3Ser. MW = 13.2 kDa.
||Protein S100-A9 is a protein that in humans is encoded by the S100A9 gene. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis.
||> 95% by SDS-PAGE. The protein was observed as a single band migrating at a molecular weight of (<20 kDa).
||0.5 mg at 1 mg/ml in Tris-HCl 10mM, NaCl 100mM pH 7.5 CaCl2 1mM. The concentration is calculated from the absorbance at 280nm (e280 = 6990M-1cm-1).
||Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated to a maximum 1mM.
||-20º C. The protein is stable at 4 ºC for at least 3 days and at 25 ºC for at least several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20º C. Avoid repeated freeze/defrost cycles.