Recombinant Human PDE4A, GST-tagged, Active

• Cat.No.: PDE4A-475H
• Product Overview: Recombinanthuman PDE4A (332-end) was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW=110kDa.

Specification

• Cat. No.: PDE4A-475H
• Description: PDE4A is a member of the phosphodiesterase family of proteins that play a critical role in regulating intracellular levels of cAMP. In vitro phosphorylation of PDE4A by the PKA-catalytic subunit increases the enzyme"s sensitivity to Mg2+, leading to a 4-fold increase in cAMP hydrolysis without affecting the Km. PDE4 is widely expressed in brain tumors and promotes their growth and treatment with PDE4A inhibitor Rolipram overcomes tumor resistance and mediates tumor regression. PDE4A expression is altered in patients with bipolar disorder and is a new therapeutic avenue for treatment of this disorder.
• Source: Sf9 insect cells using baculovirus.
• Sequence: 332-end.
• Applications: PDE Assay.
• Storage And Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• 22q13.33: 19p13.2

Gene Information

• Gene Name: PDE4A phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila) [ Homo sapiens ]
• Synonyms: PDE4A; phosphodiesterase 4A, calmodulin-dependent; cAMP-specific phosphodiesterase; phosphodiesterase 4A; phosphodiesterase 4A, cAMP-specific (dunce; phosphodiesterase E2 dunce homolog, Drosophila; phosphodiesterase isozyme 4; PDE4; DPDE2; PDE46; EC 3.1.4.17
• Gene ID: 5141
• mRNA Refseq: NM_001111307
• Protein Refseq: NP_001104777
• MIM: 600126
• UniProt ID: P27815
• Pathway: Progesterone-mediated oocyte maturation; Purine metabolism; Signaling by GPCR; Insulin signaling pathway
• Function: 3",5"-cyclic-AMP phosphodiesterase activity; cGMP-inhibited cyclic-nucleotide phosphodiesterase activity; hydrolase activity