Recombinant Human EP300, FLAG-tagged

• Cat.No.: EP300-28680TH
• Product Overview: Recombinant fragment, corresponding to C terminal amino acids 1135-2414 of Human KAT3B / p300 with Flag tag expressed in a baculovirus system.

Specification

• Description: This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.
• Conjugation: FLAG
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Contains 1 bromo domain.Contains 1 KIX domain.Contains 2 TAZ-type zinc fingers.Contains 1 ZZ-type zinc finger.

Gene Information

• Gene Name: EP300 E1A binding protein p300 [ Homo sapiens ]
• Official Symbol: EP300
• Synonyms: EP300; E1A binding protein p300; histone acetyltransferase p300; KAT3B; p300
• Gene ID: 2033
• mRNA Refseq: NM_001429
• Protein Refseq: NP_001420
• MIM: 602700
• Uniprot ID: Q09472
• Chromosome Location: 22q13.2
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BMAL1:CLOCK/NPAS2 Activates Gene Expression, organism-specific biosystem
• Function: DNA binding; NF-kappaB binding; RNA polymerase II activating transcription factor binding; RNA polymerase II core promoter proximal region sequence-specific DNA binding; acetyltransferase activity

Reviews

09/22/2022

It has consistently demonstrated outstanding results, consistently producing clear and sharp bands, allowing for accurate and reliable protein detection and quantification.

03/09/2021

Its stability and purity make it an ideal candidate for studying protein structures at a high resolution, providing crucial insights into the organization and function of the EP300 protein.

07/07/2016

I highly recommend the EP300 protein for its exceptional performance in Western blot analysis.

Q&As

How does EP300 impact developmental abnormalities?

EP300 is crucial for embryonic development, and mutations in the EP300 gene can lead to developmental abnormalities. Understanding its role is essential for potential interventions.

How does EP300 contribute to neurodegenerative disorders?

EP300 is involved in neuronal function and survival. Dysregulation of EP300 has been observed in neurodegenerative diseases like Alzheimer's and Parkinson's.

Are there experimental treatments targeting EP300 in neurodegenerative disorders?

Research is underway to explore drugs that modulate EP300 activity for potential therapeutic interventions in neurodegenerative disorders, but clinical applications are still in the early stages.

Are there any ongoing clinical trials targeting EP300 in cancer treatment?

Yes, there are clinical trials exploring the therapeutic potential of targeting EP300 in various cancer types, focusing on its role in controlling cell proliferation and survival.

Are there drugs targeting EP300 in cardiovascular diseases?

While there are no specific drugs targeting EP300 approved for cardiovascular diseases, ongoing research aims to identify compounds that can modulate its activity for therapeutic purposes.