||Recombinant human CTLA-4 encoding amino acid residue 37-161 of the extracellular domain was fused to the Fc region of human IgG1 with six his residues added at the carboxy-terminus, cloned into a suitable expression vector and was expressed inSf 21 cells.
||CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) also known as CD152, is a protein which is expressed on the surface of Helper T cells and plays an important regulatory role in the immune system. CTLA4 is a member of the immunoglobulin superfamily, expressed on the surface of Helper T cells. CTLA4 transmits an inhibitory signal to T cells. CTLA4 is potentially therapeutic in auto-immune diseases, such as rheumatoid arthritis, HIV, autoimmune thyroid disease, multiple sclerosis and may also be useful during organ transplantation and cancer treatment.
||The predicted molecular weight of Recombinant Human CTLA-4 is Mr 40 kDa.
||Sf 21 Insect Cells.
|State Of Matter:
||>90% by SDS Page and analyzed by silver stain
||<1.0 EU/µg as determined by the LAL method.
||The biological activity of Human CTLA-4 was determined by its ability to inhibit IL-2 production by Jurkat cells stimulated with Raji cells or 1 μg/ml rhB7-1/Fc in the presence of PHA. The expected ED50 is typically 2 - 4 μg/ml with Raji cells or 0.1 - 0.4 μg/ml with rhB7-H1/Fc.
|Storage And Stability:
||This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.