||Recombinant fragment, corresponding to amino acids 930-1337 of the cytoplasmic domain of Human IGF1 Receptor beta subunit fused with a proprietary tag; expressed in a baculovirus infected Sf9 cells, 72.3 kDa.
||This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
||Specific activity: ~100 nmol/min/mg at 25°C using poly(Glu:Tyr)4:1 substrate.Reaction conditions are 50 mM Tris, pH 7.5, 5 mM MgCl2, 5 mM MnCl2, 1 mM DTT, 250 μM ATP. The kinase concentration was 0.5-1 μM and poly-L-lysine was added in equimolar amounts t
||Preservative: NoneConstituents: 50mM Tris, 1mM DTT, pH 7.5
||Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.