Recombinant Human Interleukin 28A (Interferon, Lambda 2), His-tagged

• Cat.No.: IL28A-221H
• Product Overview: Recombinant Human interleukin 28A DNA sequence encoding the signal peptide from human CD33 was fused to the carboxylterminally his-tagged mature human IL-29 (Gly 20 - Thr 200) was expressed ina mouse NSO Cells.

Specification

• Cat. No.: IL28A-221H
• Description: Interleukin-28A (IL-28A) also known as IFNL2 is a novel cytokine that exerts its biological effects through a receptor that also binds IL28B and IL29. IL28A induces phosphorylation of STAT1, STAT2, and STAT3. IL-28A has antiviral activities and stimulates antiviral responses through a heterodimeric receptor. IL-28A represents a potential novel approach for therapy of Th1-mediated inflammatory diseases such as T-cell-mediated hepatitis.
• Source: Mammalian cells
• Molecular Weight: The predicted molecular weight of Recombinant Human IL-28A is Mr 20.8 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 24 kDa.
• State Of Matter: Lyophilized.
• Formulation: This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
• Purity: > 97% by SDS Page and analyzed by silver stain.
• Endotoxin: < 1.0 EU/µg as determined by the LAL method.
• Biological Activity: The biological activity of Human IFN-λ2 was determined by in an anti-viral assay using human HepG2 cells infected with encephalomyocarditis virus. The expected ED50 for this effect is typically 1 - 5 ng/mL.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Gene Information

• Gene Name: IL28A interleukin 28A (interferon, lambda 2) [ Homo sapiens ]
• Synonyms: IL28A; interleukin 28A (interferon, lambda 2);IFNL2; IL-28A; IFN-lambda-2; interferon lambda 2; interferon-lambda 2; Interleukin-28A; Interferon lambda-2; IFN-lambda-2; ZCYTO20; Cytokine ZCYTO20
• Gene ID: 282616
• mRNA Refseq: NM_172138
• Protein Refseq: NP_742150
• UniProt ID: Q8IZJ0
• Chromosome Location: 19q13.13
• MIM: 607401
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
• Function: Cytokine activity