Recombinant Human IL28A 293 Cell Lysate

Cat.No. : IL28A-5229HCL
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Description : Antigen standard for interleukin 28A (interferon, lambda 2) (IL28A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : IL28A interleukin 28A (interferon, lambda 2) [ Homo sapiens ]
Official Symbol : IL28A
Synonyms : IL28A; interleukin 28A (interferon, lambda 2); interleukin 28A; interleukin-28A; IFNL2; IL 28A; IFN-lambda-2; cytokine Zcyto20; interferon lambda 2; interferon lambda-2; interferon-lambda 2; IL-28A;
Gene ID : 282616
mRNA Refseq : NM_172138
Protein Refseq : NP_742150
MIM : 607401
UniProt ID : Q8IZJ0
Chromosome Location : 19q13.13
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem;
Function : cytokine activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the main mechanism of action of IL28A? 05/24/2021

It can enhance the body's immunity to pathogens by activating the immune system.

What is the role IL28A on the immune system? 03/11/2021

IL28A regulates the activity and differentiation of immune cells and enhances the function of the immune system.

What are the pharmacokinetic characteristics of IL28A? 03/09/2021

It has a long half-life and low clearance, which can maintain a stable plasma concentration.

How's the progress of clinical trials of IL28A? 06/20/2020

IL28A is undergoing several clinical trials and is currently showing some efficacy.

How the synthesis and purification method of IL28A? 06/19/2020

This protein is usually synthesized using recombinant DNA technology and purified by column chromatography and other methods.

What are the application prospects of IL28A in tumor therapy? 08/10/2019

IL28A can regulate immune response and enhance anti-tumor immunity in tumor therapy.

Customer Reviews (3)

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Reviews
11/15/2022

    No need to prepare a special environment, storage conditions are very simple, can be stored for a long time.

    10/04/2020

      Very stable, strong anti-pollution ability, the experimental results are very good.

      07/16/2019

        The labeling effect is very good, and fluorescent labeling can be carried out.

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