Recombinant Human RAD51, His-tagged

• Cat.No.: RAD51-29580TH
• Product Overview: Recombinant full length human RAD51 with His tag. Estimated MW 37 kDa.

Specification

• Description: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Conjugation: HIS
• Source: E. coli
• Tissue specificity: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the RecA family. RAD51 subfamily.Contains 1 HhH domain.

Gene Information

• Gene Name: RAD51 RAD51 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: RAD51
• Synonyms: RAD51; RAD51 homolog (S. cerevisiae); RAD51 (S. cerevisiae) homolog (E coli RecA homolog) , RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) , RAD51A, RECA; DNA repair protein RAD51 homolog 1; BRCA1/BRCA2 containing complex; subunit 5; BRCC5; HsRad5
• Gene ID: 5888
• mRNA Refseq: NM_001164269
• Protein Refseq: NP_001157741
• MIM: 179617
• Uniprot ID: Q06609
• Chromosome Location: 15q15.1
• Pathway: Assembly of the RAD51-ssDNA nucleoprotein complex, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem
• Function: ATP binding; ATP binding; DNA-dependent ATPase activity; damaged DNA binding; double-stranded DNA binding

Reviews

04/02/2024

RAD51-134H, Rad51 works well.

09/05/2022

User-friendly protocol included.

03/14/2022

Affordable price, great value.

01/03/2022

Product met all specifications.

Q&As

How does RAD51 contribute to the homologous recombination (HR) repair pathway?

In the HR pathway, RAD51 forms nucleoprotein filaments on single-stranded DNA, allowing it to search for and invade a homologous DNA sequence, facilitating accurate DNA repair.

Are there any known interactions between RAD51 and BRCA proteins?

Yes, RAD51 interacts with BRCA proteins, especially BRCA2. This interaction is crucial for the proper function of RAD51 in the HR pathway.

Are there any diseases or conditions associated with RAD51 dysregulation or mutations?

While mutations in RAD51 are rare, dysregulation of its function can contribute to genomic instability. Some mutations in RAD51 have been linked to increased cancer susceptibility.

What is the primary function of RAD51 in DNA repair mechanisms?

RAD51 plays a central role in the DNA repair mechanism, specifically in homologous recombination (HR), where it facilitates the search for homology and strand pairing.

How is RAD51 recruited to sites of DNA damage?

RAD51 is recruited to sites of DNA damage through interactions with various mediator proteins, including BRCA2, which helps load RAD51 onto single-stranded DNA regions.

Can RAD51 activity be targeted therapeutically in cancer treatments?

Yes, RAD51's activity can be targeted therapeutically. Inhibiting RAD51 can sensitize cancer cells to DNA-damaging agents, making it a potential target in combination cancer therapies, especially for tumors with defective DNA repair pathways.

How does RAD51's function compare to that of its paralogs, like RAD51B, RAD51C, etc.?

RAD51 has several paralogs, including RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3. While they all play roles in HR, each has distinct functions and interactions, with some acting as mediators or stabilizers of RAD51's nucleoprotein filament.