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Recombinant Human Sphingosine Kinase 1, His-tagged

Cat. No.: SPHK1-523H
Product Overview: Recombinant Human Sphingosine kinase 1 (full length) was expressed in aBaculovirus infected Sf9cell expression system with N-terminal His tag. MW=46.9 kDa.
Description: Sphingosine kinase 1 is an enzyme that in humans is encoded by the SPHK1 gene. Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1. Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor and serum.
Source: Sf9.
Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Specific Activity: 20 U/μg. Sphingosine kinase activity was determined using Derythro-sphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min. Assay buffer: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 3 μM Naorthovanadate, 0.5 mM ATP, 4 μM Derythro-sphingosine, and 0.1-0.5 μg/ml sphingosine kinase 1.
Purity: > 80%.
Stability: > 6 months at –80°C.
Gene Name: SPHK1 sphingosine kinase 1 [ Homo sapiens ]
Synonyms: sphingosine kinase 1; SPHK; SPHK1; EC 2.7.1.91; SPK; SK 1; SPK 1
Gene ID: 8877
mRNA Refseq: NM_001142601
Protein Refseq: NP_001136073
MIM: 603730
UniProt ID: Q9NYA1
Chromosome Location: 17q25.2
Pathway: Calcium signaling pathway; Fc gamma R-mediated phagocytosis; Metabolic pathways; Sphingolipid metabolism; VEGF signaling pathway
Function: ATP binding; D-erythro-sphingosine kinase activity; DNA binding; calmodulin binding; diacylglycerol kinase activity; magnesium ion binding; nucleotide binding; sphinganine kinase activity; transferase activity

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