Recombinant Mouse Sphingosine Kinase 1, His-tagged

• Cat.No.: Sphk1-524M
• Product Overview: Recombinant Mouse Sphingosine kinase 1 (full length) was expressed in a Baculovirus infected Sf9 cell expression system with N-terminal His tag. MW=47.10 kDa.

Specification

• Cat. No.: Sphk1-524M
• Description: Sphingosine kinase 1 is an enzyme that in humans is encoded by the SPHK1 gene. Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1. Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor and serum.
• Source: Baculovirus infected Sf9 cells.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
• Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Specific Activity: 20 U/ug. Sphingosine kinase activity was determined using D-erythrosphingosine and ATP as substrates. A unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min. Assay buffer: 50 mM HEPES, pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 4 uM D-erythrosphingosine, and 0.1-0.5 ug/ml sphingosine kinase 1.
• Purity: >50% by SDS-PAGE.
• Stability: > 6 months at –80°C.
• Pathways: Calcium signaling pathway; Fc gamma R-mediated phagocytosis; Metabolic pathways; Sphingolipid metabolism; VEGF signaling pathway

Gene Information

• Gene Name: Sphk1 sphingosine kinase 1 [ Mus musculus ]
• Synonyms: sphingosine kinase 1; SK1; Sphk1; 1110006G24Rik; OTTMUSP00000004201; OTTMUSP00000004202; OTTMUSP00000004203; OTTMUSP00000004204; OTTMUSP00000004205
• Gene ID: 20698
• mRNA Refseq: NM_011451
• Protein Refseq: NP_035581
• UniProt ID: Q8CI15
• Chromosome Location: 11 E2
• Function: ATP binding; DNA binding; calmodulin binding; diacylglycerol kinase activity; kinase activity; nucleotide binding; sphinganine kinase activity; transferase activity