||Recombinant human full length MART-1 protein was expressed inE.coli.
||MART-1 or MELAN-A is newly identified melanocyte differentiation antigen recognized by autologous T lymphocytes. Six other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, Tyrosinase, gp100, gp75, BAGE-1 and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum.
||> 90% by SDS-PAGE.
||250 ng in 50 ml 50 mM Tris-HCl pH 7.0 buffer containing 14 mM b-mercaptoethanol, 1 mM benzamidine, 0.1 mM phenylmethanesulfonyl fluoride, 1 mM EDTA and 0.1% Brij-35 and 10% glycerol.
|Application And Suggested Dilutions:
||•ELISA •Inhibition Assays •Western Blotting.
||On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 18kDa.
||20 µl purified human MART-1 protein at 100 µg/ml in 50 mM Tris-Acetate, pH 7.5, 1 mM EDTA and 20% Glycerol.
|Storage & Stability:
||Store vial at -20 °C to -80 °C. When stored at the recommended temperature, this protein is stable for 12 months.