Recombinant Human TNFRSF12A, Fc Chimera

• Cat.No.: TNFRSF12A-2125H
• Product Overview: The TWEAK Human Recombinant is manufactured with N-terminal fusion of 13 amino acid FLAG Tag. The TNFSF12 Flag -Tagged Fusion Protein has a Mw of 18.7kDa containing a total of 169 amino acid residues.

Specification

• Cat. No.: TNFRSF12A-2125H
• Description: TNFSF12 is a cytokine that is part of the TNF ligand family. TNFSF12 is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TNFSF12 induces apoptosis through multiple pathways of cell death in a cell type-specific manner. TNFSF12 promotes proliferation and migration of endothelial cells, and therefore acts as a regulator of angiogenesis. TNFSF12 is expressed in adult heart, pancreas, skeletal muscle, small intestine, spleen and peripheral blood lymphocytes. TWEAK h induces NFkB and chemokine secretion. TNFSF12 exerts an apoptotic activity in HT-29 human adenocarcinoma cells whilst cultured in the presence of IFN-α. TNFSF12 promotes proliferation and migration of endothelial cells.
• Source: HEK 293 cells.
• Purity: ≥90% (SDS-PAGE).
• Formulation: Filtered and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl pH-7.5.
• Solubility: Add pyrogen free water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Amino Acid Sequence: HVDYKDDDDK PAGSAPKGRK TRARRAIAAH YEVHPRPGQD GAQAGVDGTV SGWEEARINS SSPLRYNRQI GEFIVTRAGL YYLYCQVHFD EGKAVYLKLD LLVDGVLALR CLEEFSATAA SSLGPQLRLC QVSGLLALRP GSSLRIRTLP WAHLKAAPFL TYFGLFQVH.
• Applications: Western blotting.
• Stability: Stable for at least six months after receipt when stored at -20°C.

Gene Information

• Gene Name: TNFRSF12A tumor necrosis factor receptor superfamily, member 12A [ Homo sapiens ]
• Synonyms: TNFRSF12A; tumor necrosis factor receptor superfamily, member 12; FN14; CD266; TWEAKR; type I transmembrane protein Fn14; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14
• Gene ID: 51330
• mRNA Refseq: NM_016639
• Protein Refseq: NP_057723
• MIM: 605914
• UniProt ID: Q9NP84
• Chromosome Location: 16p13.3
• Pathway: Cytokine-cytokine receptor interaction
• Function: protein binding; receptor activity