Recombinant Human IFNGR1 protein, His-tagged
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|Product Overview :||Recombinant Human IFNGR1 (Glu18-Gly245) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Interferon gamma receptor 1 (IFNGR1) is also known as CD antigen CD119, which belongs to the type II cytokine receptor family. IFNGR1 contains two fibronectin type-III domains and two Ig-like C2-type (immunoglobulin-like) domains. IFNGR1/CD119 is receptor for interferon gamma. Two receptors bind one interferon gamma (IFNG) dimer.|
|Predicted N Terminal :||Glu18|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 26.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Glu18-Gly245|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of IFNGR1-2250H :||
Human IFN-gamma R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-ELISA of IFNGR1-2250H :||
Immobilized Human IFN-gamma, Tag Free at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, His Tag with a linear range of 0.01-0.313 μg/mL (Routinely tested).
|Gene Name :||IFNGR1|
|Official Symbol :||IFNGR1|
|Synonyms :||IFNGR1; interferon gamma receptor 1; IFNGR; CD119; CDw119; AVP, type 2; IFN-gamma-R1; CD119 antigen; IFN-gamma receptor 1; antiviral protein, type 2; immune interferon receptor 1; interferon-gamma receptor alpha chain; FLJ45734|
|Gene ID :||3459|
|mRNA Refseq :||NM_000416|
|Protein Refseq :||NP_000407|
|UniProt ID :||P15260|
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