Recombinant Human IFNGR1 protein, His-tagged IFNGR1-2250H

Recombinant Human IFNGR1 protein, His-tagged

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Recombinant Human IFNGR1 protein, His-tagged

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Cat.No. : IFNGR1-2250H
Product Overview : Recombinant Human IFNGR1 (Glu18-Gly245) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Interferon gamma receptor 1 (IFNGR1) is also known as CD antigen CD119, which belongs to the type II cytokine receptor family. IFNGR1 contains two fibronectin type-III domains and two Ig-like C2-type (immunoglobulin-like) domains. IFNGR1/CD119 is receptor for interferon gamma. Two receptors bind one interferon gamma (IFNG) dimer.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Glu18
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 26.6 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Glu18-Gly245
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of IFNGR1-2250H :

Human IFN-gamma R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA of IFNGR1-2250H :

Immobilized Human IFN-gamma, Tag Free at 5 μg/mL (100 μL/well) can bind Human IFN-gamma R1, His Tag with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Gene Name : IFNGR1
Official Symbol : IFNGR1
Synonyms : IFNGR1; interferon gamma receptor 1; IFNGR; CD119; CDw119; AVP, type 2; IFN-gamma-R1; CD119 antigen; IFN-gamma receptor 1; antiviral protein, type 2; immune interferon receptor 1; interferon-gamma receptor alpha chain; FLJ45734
Gene ID : 3459
mRNA Refseq : NM_000416
Protein Refseq : NP_000407
MIM : 107470
UniProt ID : P15260

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