|Cat. No. :
|Product Overview :
||Recombinant Human Melanoma Cell Adhesion Molecule(aa 433-647) is expressed inE.coli. MW=47kDa.
||CD146 belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form (118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety.
||100μl purified human CD146 protein at 100μg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide.
||ELISA, Inhibition Assays, Western Blotting.
||On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 47kDa including GST.
||Store vial at -20℃ to -80℃. When stored at the recommended temperature, this protein is stable for 12 months.