Recombinant Rat Mitogen-activated Protein Kinase 8

• Cat.No.: Mapk8-689R
• Product Overview: Recombinant rat Mapk8 is expressed inE. coli.

Specification

• Cat. No.: Mapk8-689R
• Description: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway.
• Source: E. coli.
• Form: Liquid.
• Formulation: In 150 mM NaCl, 50 mM Tris-HCl, 10 mM β-mercaptoethanol, 1 mM imidazole, 1 mM Mg (OAc) 2, 500 nM ATP, 10% glycerol, pH 7.5.
• Molar Mass: 48000Da.
• Specific Activity: ≥30 units/mg protein.
• Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1.0 nmol GST-c-Jun (1-79) Substrate per min at 30°C, pH 7.5.
• Purity: ≥90% by SDS-PAGE.
• Storage: ≤ -70oC. Ship: Dry Ice Only.

Gene Information

• Gene Name: Mapk8 mitogen-activated protein kinase 8 [ Rattus norvegicus ]
• Synonyms:
  Mapk8; mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; SAPK gamma; c-Jun N-terminal kinase 1; c-jun NH2-terminal kinase; p54 gamma; stress-activated protein kinase JNK1; JNK
• Gene ID: 116554
• mRNA Refseq: XM_341399
• Protein Refseq: XP_341400
• UniProt ID: P49185
• Chromosome Location: 16p16
• Pathway: ErbB signaling pathway; Fc epsilon RI signaling pathway; Focal adhesion; Insulin signaling pathway; NOD-like receptor signaling pathway; Neurotrophin signaling pathway; Progesterone-mediated oocyte maturation; Toll-like receptor signaling pathway; Wnt signaling pathway
• Function: ATP binding; JUN kinase activity; MAP kinase activity; protein serine/threonine kinase activity; transferase activity