Active Recombinant Human CFH protein, His-tagged

• Cat.No.: CFH-3183H
• Product Overview: Active Recombinant Human CFH protein(P08603-1)(Ser860-Arg1231) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 860-1231 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human Factor H, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Factor H Antibody, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 42.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CFH complement factor H [ Homo sapiens ]
• Official Symbol: CFH
• Synonyms: CFH; complement factor H; H factor 1 (complement) , HF, HF1, HF2; age related maculopathy susceptibility 1; ARMS1; beta 1H; FHL1; H factor 2 (complement); HUS; beta-1H; factor H; factor H-like 1; beta-1-H-globulin; H factor 1 (complement); adrenomedullin binding protein; complement factor H, isoform b; age-related maculopathy susceptibility 1; FH; HF; HF1; HF2; AHUS1; AMBP1; ARMD4; CFHL3; MGC88246
• Gene ID: 3075
• mRNA Refseq: NM_000186
• Protein Refseq: NP_000177
• MIM: 134370
• UniProt ID: P08603