Recombinant Human UDP-galactose-4-epimerase, His-tagged

• Cat.No.: GALE-731H
• Product Overview: Recombinant humanGALE protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 40.4 kDa (368aa).

Specification

• Cat. No.: GALE-731H
• Description: GALE, also known asUDP-glucose 4-epimeraes, is a protein that functions as the third enzyme in the Leloir pathway of galactose metabolism. It is a homodimeric epimerase found in bacterial, plant, and mammalian cells. This enzyme promotes the reverse chemical reaction, the conversion of UDP-glucose to UDP-galactose. UDP-galactose is used to build galactose-containing proteins and fats, which play critical roles in chemical signaling, building cellular structures, transporting molecules, and producing energy.
• Source: E.coli.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAEKVLVTGG AGYIGSHTVL ELLEAGYLPV VIDNFHNAFR GGGSLPESLR RVQELTGRSV EFEEMDILDQ GALQRLFKKY SFMAVIHFAG LKAVGESVQK PLDYYRVNLT GTIQLLEIMK AHGVKNLVFS SSATVYGNPQ YLPLDEAHPT GGCTNPYGKS KFFIEEMIRD LCQADKTWNA VLLRYFNPTG AHASGCIGED PQGIPNNLMP YVSQVAIGRR EALNVFGNDY DTEDGTGVRD YIHVVDLAKG HIAALRKLKE QCGCRIYNLG TGTGYSVLQM VQAMEKASGK KIPYKVVARR EGDVAACYAN PSLAQEELGW TAALGLDRMC EDLWRWQKQN PSGFGTQA
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 5mM DTT, 0.1M NaCl, and 1mM EDTA.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: GALE UDP-galactose-4-epimerase [ Homo sapiens ]
• Synonyms: GALE; UDP-galactose-4-epimerase; SDR1E1; FLJ95174; FLJ97302; UDP-glucose 4-epimerase; galactowaldenase; OTTHUMP00000002994; OTTHUMP00000037931; UDP-galactose 4-epimerase; UDP galactose-4"-epimerase; galactose-4-epimerase, UDP-; short chain dehydrogenase/reductase family 1E, member 1; EC 5.1.3.2
• Gene ID: 2582
• mRNA Refseq: NM_000403
• Protein Refseq: NP_000394
• MIM: 606953
• UniProt ID: Q14376
• Chromosome Location: 1p36-p35
• Pathway: Amino sugar and nucleotide sugar metabolism; Galactose metabolism; Metabolic pathways
• Function: UDP-glucose 4-epimerase activity; binding; coenzyme binding; isomerase activity; protein homodimerization activity