Recombinant Human PTK2B Protein Tyrosine Kinase 2 Beta, GST-His

• Cat.No.: PTK2B-701H
• Product Overview: Recombinant Human PTK2B, full length, amino acids M1-E1009, N-terminally fused to GST-His6-Thrombin cleavage site, was expressed inSf9insect cells.MW = 145,724 Da.

Specification

• Cat. No.: PTK2B-701H
• Description: PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family. PYK2/FAK2 can be activated by a variety of extracellular signals that elevate intracellular calcium concentration, and by stress signals. Unlike FAK, which is widely expressed in various tissues and links transmembrane integrin receptors to intracellular pathways, PYK2/FAK2 is expressed mainly in the central nervous system and in cells derived from hematopoietic lineages.
• Source: Baculovirus infected Sf9 cells.
• Product Identity: PYK2 was confirmed as PYK2 by by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
• Purification: One-step affinity purification using GSH-agarose.
• Storage Buffer: 50 mM HEPES-NaOH, pH 7.5; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
• Concentration: 0.076 μg/μl (Bradford method using BSA as standard protein).
• Specific Activity: 6 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: PTK2B PTK2B protein tyrosine kinase 2 beta [ Homo sapiens ]
• Synonyms: PTK2B; PTK2B protein tyrosine kinase 2 beta; PTK2B protein tyrosine kinase 2 beta; CAK beta; calcium-dependent tyrosine kinase; cell adhesion kinase beta; focal adhesion kinase 2; proline-rich tyrosine kinase 2; protein kinase B; protein tyrosine kinase 2 beta; related adhesion focal tyrosine kinase; EC 2.7.10.2
• Gene ID: 2185
• mRNA Refseq: NM_004103
• Protein Refseq: NP_004094
• MIM: 601212
• UniProt ID: Q14289
• Chromosome Location: 8p21.1
• Pathway: Calcium signaling pathway; Chemokine signaling pathway; GnRH signaling pathway; Leukocyte transendothelial migration; Natural killer cell mediated cytotoxicity
• Function: 3-phosphoinositide-dependent protein kinase binding; ATP binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein complex binding; signal transducer activity; transferase activity