|Cat. No. :
|Product Overview :
||Recombinant Human PLK4, full length, amino acids M1-H970, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9insect cells.MW = 138,568 Da.
||This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
|Product Identity :
||SAK was confirmed as human SAK by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
|Storage Buffer :
||50 mM HEPES, pH 7.5; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.050 μg/μl (Bradford method using BSA [Sigma, cat#A-7638, Lot 79H7641] as standard protein)
|Specific Activity :
||-80°C. Avoid repeated freeze-thaw cycles!