Recombinant Human Polo-like Kinase 4, GST-His

• Cat.No.: PLK4-705H
• Product Overview: Recombinant Human PLK4, full length, amino acids M1-H970, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9insect cells.MW = 138,568 Da.

Specification

• Cat. No.: PLK4-705H
• Description: This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle.
• Source: Baculovirus infected Sf9 cells.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: SAK was confirmed as human SAK by mass spectroscopy LC-ESI-MS/MS (Protagen AG, Germany).
• Storage Buffer: 50 mM HEPES, pH 7.5; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
• Concentration: 0.050 μg/μl (Bradford method using BSA [Sigma, cat#A-7638, Lot 79H7641] as standard protein)
• Specific Activity: 6 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: PLK4 polo-like kinase 4 [ Homo sapiens ]
• Synonyms: PLK4; polo-like kinase 4; SAK; STK18; serine/threonine-protein kinase PLK4; PLK-4; Snk akin kinase; serine/threonine kinase 18; serine/threonine-protein kinase 18; serine/threonine protein kinase SAK; serine/threonine-protein kinase Sak; EC 2.7.11.21; polo-like kinase 4 (Drosophila)
• Gene ID: 10733
• mRNA Refseq: NM_001190799
• Protein Refseq: NP_001177728
• MIM: 605031
• UniProt ID: O00444
• Chromosome Location: 4q28
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; protein tyrosine kinase activity; transferase activity