||Recombinant Human PAK2, Amino acids D3-R52, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9 cells. MW = 87,954 Da.
||The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||PAK2 was confirmed as PAK2 by specific Western Blotting using anti PAK2 antibody.
||50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.
||0.403 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!