||Recombinant Human AMPK-alpha1 Amino acids M1-Q550, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 92,188 Da.
||AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
||Baculovirus infected Sf9 cells.
||One-step affinity purification using GSH-agarose.
||AMPK-alpha1 was confirmed as human AMPK-alpha1 by mass spectroscopy LC-ESI-MS/MS.
||50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
||0.144 μg/μl (Bradford method using BSA [Sigma, cat# A-7638,Lot 79H7641] as standard protein).
||-80°C. Avoid repeated freeze-thaw cycles!