Recombinant Human PRKAA1, GST-His

• Cat.No.: PRKAA1-740H
• Product Overview: Recombinant Human AMPK-alpha1 Amino acids M1-Q550, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9cells. MW = 92,188 Da.

Specification

• Cat. No.: PRKAA1-740H
• Description: AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Source: Baculovirus infected Sf9 cells.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: AMPK-alpha1 was confirmed as human AMPK-alpha1 by mass spectroscopy LC-ESI-MS/MS.
• Storage Buffer: 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
• Concentration: 0.144 μg/μl (Bradford method using BSA [Sigma, cat# A-7638,Lot 79H7641] as standard protein).
• Specific Activity: 37 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!

Gene Information

• Gene Name: PRKAA1 protein kinase, AMP-activated, alpha 1 catalytic subunit [ Homo sapiens ]
• Synonyms: PRKAA1; protein kinase, AMP-activated, alpha 1 catalytic subunit; AMPK; AMPKa1; MGC33776; MGC57364; AMPK alpha 1; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha-1; 5"-AMP-activated protein kinase, catalytic alpha-1 chain; AMPK1EC; 2.7.11.1; AMPK alpha-1 chain, protein kinase
• Gene ID: 5562
• mRNA Refseq: NM_006251
• Protein Refseq: NP_006242
• MIM: 602739
• UniProt ID: Q13131
• Chromosome Location: 5p12
• Pathway: Adipocytokine signaling pathway; Hypertrophic cardiomyopathy (HCM); Insulin signaling pathway; Regulation of autophagy; mTOR signaling pathway
• Function: ATP binding; cAMP-dependent protein kinase activity; magnesium ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; transferase activity