Species : |
Human |
Source : |
E.coli |
Tag : |
Non |
Description : |
Fibroblast growth factor 9 (FGF9) belongs to the large FGF family which has at least 23 members. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGF-9 targets glial cells, astrocytes cells and other cells that express the FGFR 1c, 2c, 3b, 3c, and 4. |
Form : |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Bio-activity : |
The ED50 determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5 ng/mL, corresponding to a specific activity of >2.0 × 105 IU/mg. |
Molecular Mass : |
Approximately 23.4 kDa, a single non-glycosylated polypeptide chain containing 207 amino acids. |
AA Sequence : |
APLGEVGNYF GVQDAVPFGN VPVLPVDSPV LLSDHLGQSE AGGLPRGPAV TDLDHLKGIL RRRQLYCRTG FHLEIFPNGT IQGTRKDHSR FGILEFISIA VGLVSIRGVD SGLYLGMNEK GELYGSEKLT QECVFREQFE ENWYNTYSSN LYKHVDTGRR YYVALNKDGT PREGTRTKRH QKFTHFLPRP VDPDKVPELYKDILSQS |
Endotoxin : |
Less than 1 EU/μg of FGF-9 as determined by LAL method. |
Purity : |
>95% by SDS-PAGE and HPLC analyses. |
Usage : |
This material is offered by Creatvie Biomart for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE. |
Storage Buffer : |
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles. |
Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at< -20°c.="" further="" dilutions="" should="" be="" made="" in="" appropriate="" buffered=""> |