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Immobilized Human TF protein

Cat.No. : TF-338HI
Product Overview : Transferrin was from Human. The bead size is of 50-250 micron and the spacer is 3 carbon atom arm. The linkage between spacer and bead is imidoester.
  • Specification
  • Gene Information
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Description : Immobilized carbohydrates and glycoproteins have been used successfully for the selective adsorption of proteins (e.g. lectins) which have an affinity for the carbohydrate or glycoprotein that has been covalently linked to support a matrix. Purification may be carried out by applying a sample to the gel, washing through any unbound material, and eluting with a specific carbohydrate solution. The eluting carbohydrate can be removed either by dialysis or gel filtration. The gel is washed adequately to re-use the column many times. The amount of material that can be isolated in each experimental run will vary greatly and is dependent upon the molecular weight of the protein being isolated, its affinity for the particular carbohydrate linked to the gel, flow rates, and temperature.
The gel matrix is polygalactose which may cause problems with proteins having a specificity for galactose.
Source : Human
Species : Human
Form : 0.01M Phosphate - 0.15M NaCl, pH 7.2-7.4 Contains 0.05% sodium azide as a preservative.
Usage : The following information is provided only for your convenience. The success of your experiments are not guaranteed.
Procedure for Use:
1. The reaction may be carried out in a test tube (which will require centrifugation for washing steps) or in a small column (either a glass pipette or a plastic mini-column.) Gels may be run at room temperature or in a cold room. Elevated temperatures should be avoided.
2. Wash gel with 10 times the gel volume using the appropriate Buffer. Many proteins will require different buffers, pH, and ionic conditions for binding. Many will also require the addition of specific ions to insure binding. These conditions must be determined, or discovered, experimentally by the researcher.
3. Apply sample and wash unbound material from column with Buffer of your choice. DO NOT OVERWASH! The affinity for the carbohydrate/glycoprotein depends on the binding constant of the protein. Extensive washing may elute the protein to be purified if the binding constant is low.
4. Elute bound material using the appropriate carbohydrate in the Buffer of your choice. Collect small samples. Unless the optimal carbohydrate concentration has been previously determined it is recommended to start with 0.1M - 0.2M.

Procedure for Gel Regeneration:
1. After elution, wash the gel with 10 times the gel volume using 1.0-1.4 M NaCl in distilled water. Re-equilibrate the gel by washing with 50 times the gel volume using the Storage Buffer. Store refrigerated with 0.05% sodium azide as a preservative. DO NOT store the column in the high salt concentration solution. DO NOT FREEZE.
Stability : The material is stable for several years when stored refrigerated with 0.05% sodium azide added as a preservative.
Storage : Store refrigerated at 5-8 °C in Buffer. DO NOT FREEZE.
Gene Name : TF transferrin [ Homo sapiens (human) ]
Official Symbol : TF
Synonyms : TF; transferrin
Gene ID : 7018
mRNA Refseq : NM_001063.3
Protein Refseq : NP_001054.1
UniProt ID : P02787

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can TF proteins be used for diagnosing diseases? 03/19/2021

Yes, abnormalities in TF proteins can serve as diagnostic markers for certain diseases.

Are there any approved drugs that target TF proteins? 11/27/2020

Yes, some drugs target TF proteins, especially in the treatment of cancer.

How do TF proteins contribute to autoimmune diseases? 08/25/2020

TF proteins can trigger or suppress immune responses, making them relevant in understanding autoimmune diseases.

How do TF proteins play a crucial role in clinical applications? 03/16/2016

TF proteins are vital for understanding disease mechanisms and can be targeted for therapeutic purposes.

Are there any clinical tests or assays that measure TF protein levels? 03/05/2016

Yes, various assays, like ELISA and Western blot, can be used to quantify TF protein levels.

Customer Reviews (3)

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Reviews
08/01/2020

    Their team of experts is not only knowledgeable about the TF protein but is also dedicated to assisting researchers like myself in overcoming any challenges that may arise.

    01/22/2018

      This high-quality protein is crucial for the success of my experiments, ensuring that I obtain reliable and meaningful scientific findings.

      04/11/2016

        The TF protein is of exceptional quality, making it a perfect fit for my experimental needs.

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