Luciferase Reporter Gene Assay Kit

• Cat.No.: Kit-2421
• Product Overview: The Luciferase Reporter Gene Assay is based on the quantitation of luciferase expression in mammalian, yeast or E. coil cells, using luciferin and ATP as substrates. The reaction results in light production which can be conveniently measured on a luminometer. This bioluminescent reporter gene assay is extremely sensitive and is especially suitable for quantifying luciferase expression in recombinant cells. This ultra-sensitive, homogeneous cell-based assay only requires adding a single reagent to the cells and measuring the light intensity after a short incubation step (2 minutes). Assays can be performed in tubes, cuvettes or multi-well plates. All kit components are compatible with culture media and with all liquid handling systems. With an extended luminescence emission kinetics (half-life 40 min), the luciferase assays are especially suitable for high-throughput screening in 96-well, 384-well and 1536-well plates. In addition, the reagent provided in the kits has been formulated for maximum sensitivity, reproducibility and long shelf-life. Applications for this kit include gene regulation studies and high-throughput screening of gene modulators.

Specification

• Storage: 4, -20ºC
• Size: 200 tests

Reviews

05/18/2022

Worked well, happy customer.

08/30/2021

Great in lab experiments.

09/04/2020

Satisfied with the purchase.

Q&As

How is luciferase utilized in bioluminescence imaging?

It allows for real-time tracking of cellular and molecular processes in living organisms.

What is the primary function of luciferase in organisms that produce it?

Luciferase enables bioluminescence, helping organisms attract mates, prey, or deter predators.

How does luciferase produce light in a biochemical reaction?

It catalyzes the oxidation of luciferin, releasing light.

In which organisms is luciferase commonly found?

Luciferase is found in fireflies, certain jellyfish, and some fungi and bacteria.

Are there different types of luciferase, and how do they vary?

Yes, there are different types, varying in their substrate specificity and the color of emitted light.

What is the role of ATP in the luciferase reaction?

ATP provides energy for the luciferase reaction, converting luciferin to oxyluciferin.

What advantages does the use of luciferase offer in scientific research?

Luciferase provides a non-invasive and highly sensitive method for studying biological phenomena.