Recombinant Active Human IL36G Protein, His-tagged(C-ter)

• Cat.No.: IL36G-198H
• Product Overview: Recombinant Active Human IL36G Protein with His tag (C-ter) was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the interleukin 1 cytokine family. The activity of this cytokine is mediated by interleukin 1 receptor-like 2 (IL1RL2/IL1R-rp2), and is specifically inhibited by interleukin 1 family, member 5 (IL1F5/IL-1 delta). Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes. The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]
• Source: E. coli
• Species: Human
• Tag: His
• Form: Powder
• Bio-activity: Determined by its ability to induce IL-8 secretion in A431 cells. The ED50 for this effect is < 5 ng/mL.
• AA Sequence: MSMCKPITGTINDLNQQVWTLQGQN LVAVPRSDSVTPVTVAVITCKYPEA LEQGRGDPIYLGIQNPEMCLYCEKV GEQPTLQLKEQKIMDLYGQPEPVKP FLFYRAKTGRTSTLESVAFPDWFIA SSKRDQPIILTSELGKSYNTAFELN IND
• Endotoxin: Endotoxin level is less than 0.1 EU/μg of the protein, as determined by the LAL test.
• Purity: > 98% (by SDS-PAGE)
• Applications: SDS-PAGE
• Notes: For laboratory research only, not for drug, diagnostic or other use.
• Storage: Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
• Storage Buffer: PBS (pH 8.0)
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.

Gene Information

• Gene Name: IL36G interleukin 36, gamma [ Homo sapiens ]
• Official Symbol: IL36G
• Synonyms: IL36G; interleukin 36, gamma; IL1F9, interleukin 1 family, member 9; interleukin-36 gamma; IL 1F9; IL 1H1; IL 1RP2; IL1E; IL1H1; interleukin 1 related protein 2; interleukin 1 epsilon; interleukin 1 homolog 1; IL-1 epsilon; IL-1-epsilon; IL-1(EPSILON); interleukin-1 epsilon; IL-1 related protein 2; IL-1-related protein 2; interleukin-1 homolog 1; interleukin-1 family member 9; interleukin 1 family, member 9; interleukin 1-related protein 2; IL1F9; IL-1F9; IL-1H1; IL1RP2; IL-1RP2
• Gene ID: 56300
• mRNA Refseq: NM_019618
• Protein Refseq: NP_062564
• MIM: 605542
• UniProt ID: Q9NZH8

Reviews

05/29/2022

When experimenting with IL36G, the results are very reproducible.

04/14/2022

Production process of IL36G products is environmentally friendly and sustainable, in line with the concept of green production.

01/06/2022

IL36G has good solubility and ion exchange ability, which is convenient for subsequent experimental treatment and purification.

Q&As

Can IL36G treat other inflammatory diseases?

It is currently mainly used in the treatment of skin inflammatory diseases, and the therapeutic potential of other inflammatory diseases needs further study.

What are the main application areas of IL36G in scientific research?

It is mainly used in the research of the mechanism of skin inflammation and the development of new drugs.

What is the production process of IL36G?

The production of IL36G usually adopts genetic engineering technology, its genes are introduced into the expression host, and pure is obtained through fermentation, purification and other steps.

What are the advantages of IL36G in the treatment of skin inflammation compared with other drugs?

IL36G can be targeted to inhibit inflammation by directly acting on inflammation-related signaling pathways, which may be more effective than other drugs.

What is the mechanism of action of IL36G?

IL36G activates inflammation-related signaling pathways by binding to its receptors, promoting the activation of immune cells and the release of inflammatory factors.

What are the side effects of IL36G?

The side effects of IL36G may include skin irritation, allergic reactions, etc. The specific side effects can vary from individual to individual.