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Recombinant Canine IL33 Protein

Cat.No. : IL33-1034C
Product Overview : Recombinant mature form of canine IL33 (O97863) (Ser 110-Ser 263) was expressed, with an initial Met at the N-terminus.
Source : E. coli
Species : Canine
Predicted N Terminal : Met
Form : Lyophilized from sterile 5mM EDTA, PBS, pH 7.51, 5%~8% trehalose and mannitol.
Bio-activity : 1. Measured by its binding ability in a functional ELISA. Immobilized Canine IL33 at 10 μg/ml (100 μl/well) can bind human IL1R4-Fc, The EC50 of human IL1R4-Fc is 0.25-0.66 μg/ml. 2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cel
Molecular Mass : The recombinant canine IL33 consists of 155 amino acids and has a calculated molecular mass of 17.8 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 19KDa band.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >97 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : IL33 interleukin 33 [ Canis lupus familiaris ]
Official Symbol : IL33
Synonyms : DVS27; interleukin-33; IL-33
Gene ID : 403810
mRNA Refseq : NM_001003180
Protein Refseq : NP_001003180
MIM :
UniProt ID : O97863
Chromosome Location : chromosome: 11
Pathway : Cytosolic DNA-sensing pathway, organism-specific biosystem; Influenza A, organism-specific biosystem; Influenza A, conserved biosystem
Function : cytokine activity

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