Active Recombinant Cynomolgus/Rhesus CSF1 protein(Met1-Asn190), His-tagged

• Cat.No.: CSF1-339C
• Product Overview: Recombinant Cynomolgus / Rhesus CSF1 (XP_005542489.1) (Met1-Asn190) was expressed in HEK293 with a polyhistidine tag at the C-terminus. Cynomolgus and Rhesus CSF1 sequences are identical.

Specification

• Source: HEK293
• Species: Cynomolgus/Rhesus
• Tag: C-His
• Protein length: Met1-Asn190
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cell. The ED50 for this effect is typically 2-10 ng/mL.
• Molecular Mass: The recombinant cynomolgus / rhesus CSF1 consists of 169 amino acids and predicts a molecular mass of 19.8 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.