Recombinant Dog IL1B Protein

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Recombinant Dog IL1B Protein

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Cat.No. : IL1B-019D
Product Overview : Recombinant canine IL-1beta was expressed in E. coli and purified by using conventional chromatography techniques.
Description : Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Source : E.coli
Species : Dog
Form : Liquid
Bio-activity : Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 5 pg/mL.
Molecular Mass : 17.5 kDa
AA Sequence : MAAMQSVDCK LQDISHKYLV LSNSYELRAL HLNGENVNKQ VVFHMSFVHG DESNNKIPVV LGIKQKNLYL SCVMKDGKPT LQLEKVDPKV YPKRKMEKRF VFNKIEIKNT VEFESSQYPN WYISTSQVEG MPVFLGNTRG GQDITDFTME FSS
Endotoxin : < 1 EU/μg of protein (determined by LAL method)
Purity : > 95% by SDS-PAGE
Applications : SDS-PAGE, Bioactivity
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -98 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/mL (determined by absorbance at 280nm)
Storage Buffer : Phosphate-Buffered Saline (pH 7.4)
SDS-PAGE :

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Biological Activity :

Canine IL-1B stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range range ≤ 5pg/ml.

Gene Name : IL1B interleukin 1 beta [ Canis lupus familiaris (dog) ]
Official Symbol : IL1B
Synonyms : IL1B; interleukin 1 beta; IL-1; interleukin-1 beta; IL-1 beta; interleukin-1 beta proprotein
Gene ID : 403974
mRNA Refseq : NM_001037971
Protein Refseq : NP_001033060.1
UniProt ID : Q2VCE2

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