Recombinant Dog IL1B Protein
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| Cat.No. : | IL1B-019D |
| Product Overview : | Recombinant canine IL-1beta was expressed in E. coli and purified by using conventional chromatography techniques. |
| Description : | Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. |
| Source : | E.coli |
| Species : | Dog |
| Form : | Liquid |
| Bio-activity : | Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 5 pg/mL. |
| Molecular Mass : | 17.5 kDa |
| AA Sequence : | MAAMQSVDCK LQDISHKYLV LSNSYELRAL HLNGENVNKQ VVFHMSFVHG DESNNKIPVV LGIKQKNLYL SCVMKDGKPT LQLEKVDPKV YPKRKMEKRF VFNKIEIKNT VEFESSQYPN WYISTSQVEG MPVFLGNTRG GQDITDFTME FSS |
| Endotoxin : | < 1 EU/μg of protein (determined by LAL method) |
| Purity : | > 95% by SDS-PAGE |
| Applications : | SDS-PAGE, Bioactivity |
| Storage : | Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -98 centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : | 1 mg/mL (determined by absorbance at 280nm) |
| Storage Buffer : | Phosphate-Buffered Saline (pH 7.4) |
| SDS-PAGE : |
3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain |
| Biological Activity : |
Canine IL-1B stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range range ≤ 5pg/ml. |
| Gene Name : | IL1B interleukin 1 beta [ Canis lupus familiaris (dog) ] |
| Official Symbol : | IL1B |
| Synonyms : | IL1B; interleukin 1 beta; IL-1; interleukin-1 beta; IL-1 beta; interleukin-1 beta proprotein |
| Gene ID : | 403974 |
| mRNA Refseq : | NM_001037971 |
| Protein Refseq : | NP_001033060.1 |
| UniProt ID : | Q2VCE2 |
◆ Recombinant Protein
| Il1b-100M | Recombinant Mouse Il1b Protein | +Inquiry |
| Il1b-102M | Recombinant Mouse Il1b Protein | +Inquiry |
| IL1B-920C | Recombinant Canine IL1B Protein (Ala115-Ser266) | +Inquiry |
| IL1B-132H | Recombinant Human IL1B Protein | +Inquiry |
| Il1b-101M | Recombinant Mouse Il1b Protein | +Inquiry |
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◆ Lysates
| IL1B-853HCL | Recombinant Human IL1B cell lysate | +Inquiry |
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IL10IL10RAIL10RBIL11IL11RAIl11ra1IL12IL12AIL12BIL12RB1IL13IL13RA1IL13RA2IL15IL15RAIL16IL17AIL17BIl17cIL17DIl17fIL17RAIL17RDIL18IL18BPIL18R1IL18RAPIl19Il1aIl1bIL1F10IL1R1Il1r2IL1RAPIL1RAPL1IL1RAPL2IL1RL1IL1RL2Il1rnIL2Il20IL20RAIL21IL21RIL22IL22BPIL22RA1IL22RA2IL23AIL24Il25IL27IL27RAIL28AIl28bIL29IL2RAIL2RBIl2rgIL3IL31IL32Il33Il34IL3RAIL4IL4RIl4raIl5IL5RAIl6IL6RIl6raIL6STIl7IL7RIL8IL9IL36AIl12rb2IL17RBIL23RIL26IL31RAIL36BIL36GIL36RNIL37IL9RIL17RCIL17REIL28RAIL4I1IL20RBIL17RELIl11ra2IL27bIL7RAIl23a&Il12b
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