Recombinant Full Length Human AKR1C3 Protein, C-Flag-tagged

• Cat.No.: AKR1C3-1971HFL
• Product Overview: Recombinant Full Length Human AKR1C3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 36.7 kDa
• AA Sequence: MDSKHQCVKLNDGHFMPVLGFGTYA PPEVPRSKALEVTKLAIEAGFRHID SAHLYNNEEQVGLAIRSKIA DGSVKREDIFYTSKLWSTFHRPELV RPALENSLKKAQLDYVDLYLIHSPM SLKPGEELSPTDENGKVIFD IVDLCTTWEAMEKCKDAGLAKSIGV SNFNRRQLEMILNKPGLKYKPVCNQ VECHPYFNRSKLLDFCKSKD IVLVAYSALGSQRDKRWVDPNSPVL LEDPVLCALAKKHKRTPALIALRYQ LQRGVVVLAKSYNEQRIRQN VQVFEFQLTAEDMKAIDGLDRNLHY FNSDSFASHPNYPYSDEY TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Metabolism of xenobiotics by cytochrome P450

Gene Information

• Gene Name: AKR1C3 aldo-keto reductase family 1 member C3 [ Homo sapiens (human) ]
• Official Symbol: AKR1C3
• Synonyms: DD3; DDX; PGFS; HAKRB; HAKRe; HA1753; HSD17B5; hluPGFS
• Gene ID: 8644
• mRNA Refseq: NM_003739.6
• Protein Refseq: NP_003730.4
• MIM: 603966
• UniProt ID: P42330