Recombinant Full Length Human CA3 Protein, C-Flag-tagged

• Cat.No.: CA3-926HFL
• Product Overview: Recombinant Full Length Human CA3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 29.4 kDa
• AA Sequence: MAKEWGYASHNGPDHWHELFPNAKG ENQSPIELHTKDIRHDPSLQPWSVS YDGGSAKTILNNGKTCRVVF DDTYDRSMLRGGPLPGPYRLRQFHL HWGSSDDHGSEHTVDGVKYAAELHL VHWNPKYNTFKEALKQRDGI AVIGIFLKIGHENGEFQIFLDALDK IKTKGKEAPFTKFDPSCLFPACRDY WTYQGSFTTPPCEECIVWLL
  LKEPMTVSSDQMAKLRSLLSSAENE PPVPLVSNWRPPQPINNRVVRASFK TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Nitrogen metabolism

Gene Information

• Gene Name: CA3 carbonic anhydrase 3 [ Homo sapiens (human) ]
• Official Symbol: CA3
• Synonyms: Car3; CAIII
• Gene ID: 761
• mRNA Refseq: NM_005181.4
• Protein Refseq: NP_005172.1
• MIM: 114750
• UniProt ID: P07451

Reviews

09/23/2022

Outstanding protein sequencing capabilities, unrivaled accuracy.

05/23/2022

Precise purification techniques, yield exceptional samples.

05/25/2021

Efficient sample handling, optimizes our laboratory workflow.

Q&As

In which tissues is CA3 expression most prominently observed?

CA3 is primarily found in skeletal muscles.

What post-translational modifications are characteristic of CA3?

Post-translational modifications, like phosphorylation or glycosylation, can be identified using mass spectrometry.

Are there known inhibitors that specifically target CA3?

Specific inhibitors for CA3 are yet to be fully elucidated and would be determined using enzymatic assays.

How does CA3 participate in metabolic processes within the cell?

CA3 plays a role in CO2 and bicarbonate equilibrium, crucial for metabolic pathways like glycolysis and the citric acid cycle.

How does CA3 enzymatic activity differ from other carbonic anhydrase isoforms?

While CA3 has a similar mechanism, its activity and affinity might differ from other isoforms, requiring comparative studies.

Are there any pathological conditions directly linked to CA3 dysregulation?

CA3 dysregulation might be linked to muscle diseases or metabolic disorders, requiring further research.

What is the primary physiological role of CA3 in cells?

CA3 catalyzes the reversible hydration of carbon dioxide, aiding in pH regulation and CO2 transport.