Recombinant Full Length Human HMGCS2 Protein, C-Flag-tagged

• Cat.No.: HMGCS2-1766HFL
• Product Overview: Recombinant Full Length Human HMGCS2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 52.3 kDa
• AA Sequence: MQRLLTPVKRILQLTRAVQETSLTP ARLLPVAHQRFSTASAVPLAKTDTW PKDVGILALEVYFPAQYVDQ TDLEKYNNVEAGKYTVGLGQTRMGF CSVQEDINSLCLTVVQRLMERIQLP WDSVGRLEVGTETIIDKSKA VKTVLMELFQDSGNTDIEGIDTTNA CYGGTASLFNAANWMESSSWDGRYA MVVCGDIAVYPSGNARPTGG AGAVAMLIGPKAPLALERGLRGTHM ENVYDFYKPNLASEYPIVDGKLSIQ CYLRALDRCYTSYRKKIQNQ WKQAGSDRPFTLDDLQYMIFHTPFC KMVQKSLARLMFNDFLSASSDTQTS LYKGLEAFGGLKLEDTYTNK DLDKALLKASQDMFDKKTKASLYLS THNGNMYTSSLYGCLASLLSHHSAQ ELAGSRIGAFSYGSGLAASF FSFRVSQDAAPGSPLDKLVSSTSDL PKRLASRKCVSPEEFTEIMNQREQF YHKVNFSPPGDTNSLFPGTW YLERVDEQHRRKYARRPV TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Metabolic pathways, PPAR signaling pathway, Synthesis and degradation of ketone bodies, Terpenoid backbone biosynthesis, Valine, leucine and isoleucine degradation

Gene Information

• Gene Name: HMGCS2 3-hydroxy-3-methylglutaryl-CoA synthase 2 [ Homo sapiens (human) ]
• Official Symbol: HMGCS2
• Synonyms: 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial); hydroxymethylglutaryl-CoA synthase 2; OTTHUMP00000013928
• Gene ID: 3158
• mRNA Refseq: NM_005518.4
• Protein Refseq: NP_005509.1
• MIM: 600234
• UniProt ID: P54868