Recombinant Human CD82 Protein, C-His-tagged

• Cat.No.: CD82-146H
• Product Overview: Recombinant Human CD82 Protein with C-His tag was expressed in E. coli.

Specification

• Description: CD82 (KAI1) belongs to the tetraspanin family, which is characterized by four transmembrane domains, one short extracellular domain (ECL1), and one long extracellular domain (ECL2). CD82 does not have enzymatic activity and appears to function by regulating the trafficking of other proteins and organization of the cell membrane. CD82 was originally described as a costimulator for T cells that directly associates with CD4 and CD8, and was subsequently identified during a screen as a metastasis suppressor in prostate cancer. CD82 has since been found to act as a metastasis suppressor in a variety of cancers, and its downregulation is associated with poor prognosis in research studies. CD82 suppresses metastasis through multiple mechanisms including inhibition of cell motility and invasion by modulating c-Met and the urokinase plasminogen activator surface receptor (uPAR), as well as promotion of homotypic cell-cell adhesion by stabilizing interactions between E-cadherin and β-catenin.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: ~13 kDa
• AA Sequence: GKLKQEMGGIVTELIRDYNSSREDS LQDAWDYVQAQVKCCGWVSFYNWTD NAELMNRPEVTYPCSCEVKGEEDNS LSVRKGFCEAPGNRTQSGNHPEDWP VYQEGCMEKVQAWLQENL
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).
• Notes: For research use only, not for use in diagnostic procedure.
• Storage: Store at 4 centigrade short term. Aliquot and store at -20 centigrade long term. Avoid freeze-thaw cycles.
• Concentration: ≥0.5 mg/mL
• Storage Buffer: PBS, 4M Urea, pH7.4

Gene Information

• Gene Name: CD82 CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal [ Homo sapiens (human) ]
• Official Symbol: CD82
• Synonyms: CD82; CD82 antigen (R2 leukocyte antigen, antigen detected; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal; SAR2
• Gene ID: 3732
• mRNA Refseq: NM_002231
• Protein Refseq: NP_002222
• MIM: 600623
• UniProt ID: P27701

Reviews

02/28/2021

Integral to our research. Highly recommended service.

10/22/2020

Exceptional accuracy and support. A research necessity.

08/26/2018

Boosts research efficiency. Contributes to discoveries.

Q&As

How do genetic variations in CD82 affect cancer progression?

Genetic mutations in CD82 can lead to reduced tumor-suppressive functions, enhancing the risk of cancer spread.

What potential therapeutic applications arise from targeting CD82 in cancer treatment?

Targeting CD82 in cancer therapy could exploit its tumor-suppressive properties to prevent metastasis.

How does CD82 interact with other membrane proteins in signaling pathways?

CD82 collaborates with other membrane proteins to regulate various signaling pathways, impacting cell behavior.

What is the primary role of CD82 in cell signaling and communication?

CD82, a member of the tetraspanin family, is involved in modulating cell signaling and intercellular communication.

What is the impact of altered CD82 activity on immune response?

Altered CD82 activity can influence immune cell interactions and responses.

What role does CD82 play in tumor suppression and metastasis prevention?

CD82 acts as a tumor suppressor, inhibiting cancer cell migration and metastasis.

How does CD82 contribute to the regulation of cell migration and adhesion?

It plays a crucial role in regulating cell migration and adhesion, key processes in tissue organization and wound healing.