Recombinant Human CFH, His tagged

• Cat.No.: CFH-3183H
• Product Overview: Recombinant Human CFH (NP_000177.2) C-terminal segment corresponding to amino acid (Ser 860-Arg 1231), fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus, was produced in Human Cell.

Specification

• Source: Human Cells
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The recombinant human CFH consists of 383 amino acids and has a calculated molecular mass of 43 kDa. The apparent molecular mass of rh CFH is approximately 55-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: Ser860-Arg1231

Gene Information

• Gene Name: CFH complement factor H [ Homo sapiens ]
• Official Symbol: CFH
• Gene ID: 3075
• mRNA Refseq: NM_000186
• Protein Refseq: NP_000177
• MIM: 134370
• UniProt ID: P08603

Reviews

11/16/2020

Exceptional protein expression optimization support, highly appreciated assistance.

07/31/2020

Precise protein quantification, ensures data accuracy in our research.

03/10/2018

Timely antibody validation, an invaluable asset for our laboratory.

Q&As

What is the impact of CFH mutations on the risk of developing atypical hemolytic uremic syndrome (aHUS)?

Mutations in CFH increase the risk of aHUS, affecting complement regulation and leading to kidney damage.

How does CFH (Complement Factor H) regulate the complement system and prevent overactivation?

CFH controls the complement system, preventing its overactivation and protecting against immune damage.

How does CFH influence the immune response to bacterial infections?

CFH modulates the immune response to bacterial infections, balancing defense mechanisms.

How does CFH deficiency contribute to the development of age-related macular degeneration (AMD)?

CFH deficiency is linked to AMD, due to impaired regulation of the complement pathway in the retina.

What role does CFH play in protecting host cells from complement-mediated damage?

CFH shields host cells from complement-mediated injury, maintaining tissue integrity.

What are the mechanisms by which CFH modulates inflammation and immune homeostasis?

CFH regulates inflammation and maintains immune balance, crucial for preventing excessive immune responses.

How do alterations in CFH expression or function affect renal function and the development of kidney diseases?

Changes in CFH function can impact kidney health, potentially leading to renal diseases due to altered complement activity.