Recombinant Human DHFR Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human DHFR Protein, Myc/DDK-tagged, C13 and N15-labeled

DHFR Related Products

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Cat.No. : DHFR-6261H
Product Overview : DHFR MS Standard C13 and N15-labeled recombinant protein (NP_000782) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 21.5 kDa
AA Sequence : MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKNDTRT RPLEQKLISE EDLAANDILD YKDDDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : DHFR dihydrofolate reductase [ Homo sapiens (human) ]
Official Symbol : DHFR
Synonyms : DHFR; dihydrofolate reductase; DYR; DHFRP1;
Gene ID : 1719
mRNA Refseq : NM_000791
Protein Refseq : NP_000782
MIM : 126060
UniProt ID : P00374

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