Active Recombinant Human EGFR protein, His-Avi-tagged, FITC-Labeled

• Cat.No.: EGFR-54H
• Product Overview: FITC-Labeled Active Recombinant Human EGFR protein(NP_001333870.1)(Leu25-Ser378) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 25-378 aa
• Conjugation/Label: FITC
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Wavelength: Excitation Wavelength: 490 nm Emission Wavelength: 520 nm
• Bio-activity: Immobilized FITC-Labeled Human EGFRVIII, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-EGFRVIII Antibody, hFc Tag with the EC50 of 1.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Concentration: 0.1-1.0 mg/mL. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: EGFR epidermal growth factor receptor [ Homo sapiens ]
• Official Symbol: EGFR
• Synonyms: EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) , ERBB; ERBB1; erythroblastic leukemia viral (v erb b) oncogene homolog (avian); proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; ERBB; HER1; mENA; PIG61
• Gene ID: 1956
• mRNA Refseq: NM_005228
• Protein Refseq: NP_005219
• MIM: 131550
• UniProt ID: P00533