Recombinant Human EIF5 Protein (1-431 aa), His-SUMO-tagged

• Cat.No.: EIF5-478H
• Product Overview: Recombinant Human EIF5 Protein (1-431 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Full Length.

Specification

• Description: Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 65.2 kDa
• Protein length: 1-431 aa
• AA Sequence: MSVNVNRSVSDQFYRYKMPRLIAKV EGKGNGIKTVIVNMVDVAKALNRPP TYPTKYFGCELGAQTQFDVKNDRYI VNGSHEANKLQDMLDGFIKKFVLCP ECENPETDLHVNPKKQTIGNSCKAC GYRGMLDTHHKLCTFILKNPPENSD SGTGKKEKEKKNRKGKDKENGSVSS SETPPPPPPPNEINPPPHTMEEEED DDWGEDTTEEAQRRRMDEISDHAKV LTLSDDLERTIEERVNILFDFVKKK KEEGVIDSSDKEIVAEAERLDVKAM GPLVLTEVLFNEKIREQIKKYRRHF LRFCHNNKKAQRYLLHGLECVVAMH QAQLISKIPHILKEMYDADLLEEEV IISWSEKASKKYVSKELAKEIRVKA EPFIKWLKEAEEESSGGEEEDEDEN IEVVYSKAASVPKVETVKSDNKDDD IDIDAI
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: EIF5 eukaryotic translation initiation factor 5 [ Homo sapiens ]
• Official Symbol: EIF5
• Synonyms: EIF5; eIF-5; EIF-5A
• Gene ID: 1983
• mRNA Refseq: NM_001969
• Protein Refseq: NP_001960
• MIM: 601710
• UniProt ID: P55010