FGF9-16H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant human FGF-9 protein without tag was expressed in E. coli. Animal-derived component free (ACDF) and carrier protein-free. Mass spectrometry: single species with expected mass. Analytical reversed-phase: single sharp peak. Recovery from stock vial: >95%
Description :
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis.
Source :
E. coli
Species :
Human
Molecular Mass :
23 kDa (monomer)
Endotoxin :
<0.005 EU/μg protein (below level of detection)
Applications :
Kidney organoid generation; Chemically defined media
Storage :
Resuspend in water at >100 μg/mL, prepare single use aliquots, add carrier protein if desired and store frozen at -20 or -80 centigrade
Storage Buffer :
Lyophilized from PBS
Bioactivity :
FGF9 activity is determined using the serum response element luciferase reporter assayin transfected HEK293T cells. EC50 = 5.7 ng/mL (0.24 nM). Cells are treated in triplicate with a serial dilution of FGF-9 for 3 hours. Firefly luciferase activity is measured and normalized to the control Renilla luciferase activity.
Purity :
FGF-9 migrates as a single band at 23 kDa in non-reducingconditions and upon reductionwas resolved using 15% w/v SDS-PAGE in reducedand non-reducedconditions and stained with Coomassie Brilliant Blue R250.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.