Active Recombinant Human FLT3 protein, hFc-tagged, Low Endotoxin

• Cat.No.: FLT3-4411H
• Product Overview: Recombinant Human FLT3 protein(P36888-1)(Asn27-Asn541), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Protein Length: 27-541 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human FLT3 Ligand, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 84.9 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: FLT3 fms-related tyrosine kinase 3 [ Homo sapiens ]
• Official Symbol: FLT3
• Synonyms: FLT3; fms-related tyrosine kinase 3; receptor-type tyrosine-protein kinase FLT3; CD135; FLK2; STK1; STK-1; CD135 antigen; FL cytokine receptor; fetal liver kinase 2; fms-like tyrosine kinase 3; stem cell tyrosine kinase 1; growth factor receptor tyrosine kinase type III; FLK-2
• Gene ID: 2322
• mRNA Refseq: NM_004119
• Protein Refseq: NP_004110
• MIM: 136351
• UniProt ID: P36888