Recombinant Human GALC Protein (43-685 aa), His-tagged

• Cat.No.: GALC-1844H
• Product Overview: Recombinant Human GALC Protein (43-685 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Cardiovascular. Protein Description: Full Length of Mature Protein.

Specification

• Description: Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon.
• Source: Yeast
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 74.8 kDa
• Protein length: 43-685 aa
• AA Sequence: YVLDDSDGLGREFDGIGAVSGGGAT SRLLVNYPEPYRSQILDYLFKPNFG ASLHILKVEIGGDGQTTDGTEPSHM HYALDENYFRGYEWWLMKEAKKRNP NITLIGLPWSFPGWLGKGFDWPYVN LQLTAYYVVTWIVGAKRYHDLDIDY IGIWNERSYNANYIKILRKMLNYQG LQRVKIIASDNLWESISASMLLDAE LFKVVDVIGAHYPGTHSAKDAKLTG KKLWSSEDFSTLNSDMGAGCWGRIL NQNYINGYMTSTIAWNLVASYYEQL PYGRCGLMTAQEPWSGHYVVESPVW VSAHTTQFTQPGWYYLKTVGHLEKG GSYVALTDGLGNLTIIIETMSHKHS KCIRPFLPYFNVSQQFATFVLKGSF SEIPELQVWYTKLGKTSERFLFKQL DSLWLLDSDGSFTLSLHEDELFTLT TLTTGRKGSYPLPPKSQPFPSTYKD DFNVDYPFFSEAPNFADQTGVFEYF TNIEDPGEHHFTLRQVLNQRPITWA ADASNTISIIGDYNWTNLTIKCDVY IETPDTGGVFIAGRVNKGGILIRSA RGIFFWIFANGSYRVTGDLAGWIIY ALGRVEVTAKKWYTLTLTIKGHFAS GMLNDKSLWTDIPVNFPKNGWAAIG THSFEFAQFDNFLVEATR
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: GALC galactosylceramidase [ Homo sapiens ]
• Official Symbol: GALC
• Synonyms: GALC; galactosylceramidase; galactocerebrosidase; Krabbe disease; GALCERase
• Gene ID: 2581
• mRNA Refseq: NM_000153
• Protein Refseq: NP_000144
• MIM: 606890
• UniProt ID: P54803