Recombinant Human GOT1 protein, His-tagged

• Cat.No.: GOT1-434H
• Product Overview: Recombinant Human GOT1 (P17174) aa. (Ala2~Gln413) fused with His tag was produced in Yeast cells.

Specification

• Species: Human
• Source: Yeast
• Tag: His
• Protein Length: Ala2~Gln413
• Description: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 47.5kDa; Accurate Molecular Mass: 48kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: Isoelectric point is 6.6.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200ug/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: GOT1 glutamic-oxaloacetic transaminase 1 [ Homo sapiens (human) ]
• Official Symbol: GOT1
• Synonyms: GOT1; glutamic-oxaloacetic transaminase 1; AST1; cCAT; GIG18; cAspAT; ASTQTL1; aspartate aminotransferase, cytoplasmic; aspartate aminotransferase 1; aspartate transaminase 1; cysteine aminotransferase, cytoplasmic; cysteine transaminase, cytoplasmic; glutamate oxaloacetate transaminase 1; glutamic-oxaloacetic transaminase 1, soluble; growth-inhibiting protein 18; testis secretory sperm-binding protein Li 196a; transaminase A
• Gene ID: 2805
• mRNA Refseq: NM_002079.2
• Protein Refseq: NP_002070.1
• UniProt ID: P17174