| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Protein Length : |
320-454 a.a. |
| Description : |
Growth/differentiation factor 9 is a protein that in humans is encoded by the GDF9 gene. Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. Growth differentiation factor-9 (GDF9) is expressed in oocytes and is thought to be required for ovarian folliculogenesis. GDF9 is a member of the transforming growth factor-beta (TGFβ) superfamily. GDF9 plays an important role in the development of primary follicles in the ovary. It has a critical role in granulosa cell and theca cell growth, as well as in differentiation and maturation of the oocyte. |
| Sequence : |
Human Growth differentiation factor (Gln320-Arg454P)320GQETVSSELK KPLGPASFNL SEYFRQFLLP QNECELHDFR LSFSQLKWDN WIVAPHRYNP RYCKGDCPRA VGHRYGSPVH TMVQNIIYEK LDSSVPRPSC VPAKYSPLSV LTIEPDGSIA YKEYEDMIAT KCTCR 454. |
| Purity : |
>95% in SDS gel. |
| Formulation : |
Lyophilized 100 μg human Growth differentiation factor 9 in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free. |
| Application : |
Cell biology, ELISA. |
| Reconstitution : |
Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well. |
| Storage : |
Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles. |
