Recombinant Human Growth Differentiation Factor 9, His-tagged

• Cat.No.: GDF9-223H
• Product Overview: Recombinant Human Growth Differentiation Factor 9 encoding the mature human Growth differentiation factor 9 (Gln320-Arg454P) was recombined with his tag at the N-Terminus. That was expressed inE.Coli, and purified by Ni-NTA column. MW=14.9KD.

Specification

• Cat. No.: GDF9-223H
• Description: Growth/differentiation factor 9 is a protein that in humans is encoded by the GDF9 gene. Growth factors synthesized by ovarian somatic cells directly affect oocyte growth and function. Growth differentiation factor-9 (GDF9) is expressed in oocytes and is thought to be required for ovarian folliculogenesis. GDF9 is a member of the transforming growth factor-beta (TGFβ) superfamily. GDF9 plays an important role in the development of primary follicles in the ovary. It has a critical role in granulosa cell and theca cell growth, as well as in differentiation and maturation of the oocyte.
• Source: E. Coli.
• Sequence: Human Growth differentiation factor (Gln³²⁰-Arg^454P)320GQETVSSELK KPLGPASFNL SEYFRQFLLP QNECELHDFR LSFSQLKWDN WIVAPHRYNP RYCKGDCPRA VGHRYGSPVH TMVQNIIYEK LDSSVPRPSC VPAKYSPLSV LTIEPDGSIA YKEYEDMIAT KCTCR 454.
• Purity: >95% in SDS gel.
• Formulation: Lyophilized 100 μg human Growth differentiation factor 9 in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
• Application: Cell biology, ELISA.
• Reconstitution: Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
• Storage: Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
• Protein length: Gln320-Arg454P

Gene Information

• Gene Name: GDF9 growth differentiation factor 9 [ Homo sapiens ]
• Synonyms: GDF9; growth differentiation factor 9; OTTHUMP00000066213;GDF-9; Growth/differentiation factor 9
• Gene ID: 2661
• mRNA Refseq: NM_005260
• Protein Refseq: NP_005251
• MIM: 601918
• UniProt ID: O60383
• Chromosome Location: 5q31.1
• Function: cytokine activity; growth factor activity

Reviews

11/14/2021

Expertise in peptide synthesis, accelerates our peptide-related projects.

08/24/2020

Swift protein quantification, improves data reliability in our experiments.

01/31/2019

Outstanding protein-protein interaction studies, boost our research efforts.

Q&As

How does GDF9 influence the proliferation and differentiation of granulosa cells in the ovary?

GDF9 stimulates granulosa cell proliferation and differentiation, essential for healthy ovarian function.

What are the mechanisms by which GDF9 modulates intra-ovarian signaling pathways?

GDF9 interacts with intra-ovarian signaling pathways, coordinating follicle development and ovulation.

How do alterations in GDF9 expression or function affect embryonic development and early pregnancy?

Changes in GDF9 expression impact embryonic development and the success of early pregnancy stages.

What role does GDF9 play in fertility and reproductive outcomes in females?

GDF9 significantly impacts female fertility, affecting egg quality and reproductive potential.

What is the impact of GDF9 mutations on the pathogenesis of ovarian dysfunctions and disorders?

Mutations in GDF9 can lead to ovarian disorders, impacting follicle development and female fertility.

How does GDF9 regulate ovarian follicle development and oocyte maturation?

GDF9 is crucial for ovarian follicle growth and oocyte maturation, influencing female fertility.

How does GDF9 contribute to the regulation of steroidogenesis in ovarian cells?

GDF9 regulates steroid hormone production in ovarian cells, influencing reproductive hormone balance.