Recombinant Human HDAC1 Protein, His/FLAG-tagged

• Cat.No.: HDAC1-45H
• Product Overview: Recombinant human HDAC1 protein with a C-terminal His/FLAG-tag was expressed in Bacmid/Sf21 cells.

Specification

• Description: Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
• Source: Insect cells
• Species: Human
• Tag: His/FLAG
• Molecular Mass: 57 kDa
• Purity: ≥55% estimated by SDS-PAGE
• Unit Definition: One unit is the amount of enzyme required to release 1 pmol of acetate per minute at 37 centigrade in 25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/mL BSA, and 20 μM fluorogenic HDAC substrate 3 and HDAC1.
• Stability: ≥ 6 months
• Storage: Store it at -80 centigrade
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, with 110 mM sodium chloride, 2.2 mM potassium chloride and 20% glycerol

Gene Information

• Gene Name: HDAC1 histone deacetylase 1 [ Homo sapiens (human) ]
• Official Symbol: HDAC1
• Synonyms: HDAC1; histone deacetylase 1; HD1; RPD3; KDAC1; GON-10; RPD3L1; histone deacetylase 1; reduced potassium dependency, yeast homolog-like 1
• Gene ID: 3065
• mRNA Refseq: NM_004964
• Protein Refseq: NP_004955
• MIM: 601241
• UniProt ID: Q13547