Active Recombinant Human IL12A/IL12B protein
Cat.No. : | IL12A-27H |
Product Overview : | The hIL-12 consists of two subunits linked via a disulphide bond: P35 (Accession# NP_000873.2: Arg 57- Ser 253) and P40 (Accession# NP_002178.2: Ile 23-Ser 328) was expressed in insect cells as secreted protein (p35, p40). |
- Specification
- Gene Information
- Related Products
Source : | Insect cell |
Species : | Human |
Form : | The protein was 0.22μm filtered in 10mM NaH2PO4, 150mM NaCl, pH 7.2. |
Bio-activity : | ED50 = 0.05 - 0.25 ng/ml, corresponding to a specific activity of 0.4 - 2.0 x 10^7 units/mg, as determined by the production of IFNγ by activated human PBMC in response to IL-12. |
Molecular Mass : | The total predicted molecular weight is 57 kDa. The non-reduced protein migrates at approximately 55 kDa and the DTT-reduced protein produces two bands migrating at approximately 26 kDa and 40 KDa by SDS-PAGE. |
AA Sequence : | RNLPVATPDPGMFPCLHHSQNLLRA VSNMLQKARQTLEFYPCTSEEIDHE DITKDKTSTVEACLPLELTKNESCL NSRETSFITNGSCLASRKTSFMMAL CLSSIYEDLKMYQVEFKTMNAKLLM DPKRQIFLDQNMLAVIDELMQALNF NSETVPQKSSLEEPDFYKTKIKLCI LLHAFRIRAVTIDRVMSYLNAS p40 Subunit IWELKKDVYVVELDWYPDAPGEMVV LTCDTPEEDGITWT |
Endotoxin : | < 1 EU/µg determined by LAL method |
Purity : | >95 % as determined by SDS-PAGE analysis |
Applications : | ELISA, WB, Antibody Production, Protein array, Bioassay |
Storage : | Unopened vial can be stored at -20°C for six months or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10µg/mL in buffer containing carrier protein such as 1% BSA or HSA or 1 |
Gene Name : | IL12A interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35) [ Homo sapiens ] |
Official Symbol : | IL12A |
Synonyms : | IL12A; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); NKSF1; interleukin-12 subunit alpha; CLMF; cytotoxic lymphocyte maturation factor 1; p35; IL 12; subunit p35; IL 12A; IL35 subunit; interleukin 12; interleukin 12 alpha chain; natural killer cell stimulatory factor 1; 35 kD subunit; NF cell stimulatory factor chain 1; NFSK; CLMF p35; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin-12 alpha chain; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; natural killer cell stimulatory factor 1, 35 kD subunit; P35; IL-12A; |
Gene ID : | 3592 |
mRNA Refseq : | NM_000882 |
Protein Refseq : | NP_000873 |
MIM : | 161560 |
UniProt ID : | P29459 |
Chromosome Location : | 3p12-q13.2 |
Pathway : | African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; |
Function : | contributes_to cytokine activity; contributes_to cytokine activity; contributes_to growth factor activity; contributes_to growth factor activity; interleukin-12 beta subunit binding; interleukin-12 receptor binding; interleukin-27 binding; protein binding |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionMass spectrometry identifies IL12A's post-translational modifications and their roles.
Knockout models validate IL12A's roles in immune response modulation.
Protein footprinting assays reveal IL12A's binding interfaces with receptors.
ELISA and stability assays quantify IL12A secretion and stability in culture.
SPR characterizes IL12A's interactions with antibodies and inhibitors.
Cellular assays gauge IL12A's cytokine induction in immune cells.
Isothermal titration calorimetry measures IL12A's binding affinity with receptors.
Structural biology techniques analyze IL12A genetic variant impacts.
Site-directed mutagenesis probes IL12A's functional domains and interactions.
Bioluminescence imaging tracks IL12A expression dynamics in vivo.
Customer Reviews (3)
Write a reviewSuitable for co-immunoprecipitation.
Consistent in bioactivity.
Good for receptor binding studies.
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