Active Recombinant Human IL17C protein

• Cat.No.: IL17C-185H
• Product Overview: Recombinant Human IL17C(His19-Val197) fused with Met at N-terminal was expressed in E. coli.

Specification

• Description: Interleukin‑17C (IL‑17C) is a 15‑20 kDa glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold. Mature human IL‑17C shares 79% and 76% amino acid sequence identity with mouse and rat IL‑17C, respectively. IL‑17C binds to IL‑17 RE with high affinity and to IL‑17 RA with low affinity. These two receptor chains can associate into a heterodimeric receptor for IL‑17C. IL‑17 RE is expressed on keratinocytes, mucosal epithelial cells, Th17 cells, and gamma /δ T cells, while IL‑17 RA is widely expressed. IL‑17 RE is required for mediating the pro‑inflammatory and homeostatic actions of IL‑17C in the skin and mucosa. IL‑17C expression is induced by inflammatory stimulation in colon and airway epithelial cells, keratinocytes, CD4+ T cells, macrophages, and dendritic cells. It is up‑regulated in various chronic inflammatory diseases including psoriasis, cystic fibrosis, and chronic obstructive pulmonary disease (COPD). IL‑17 RE is reciprocally down‑regulated in psoriatic lesions (10). The interaction of IL‑17C with IL‑17 RE promotes mucosal immunity through the induction of anti‑bacterial peptides and pro‑inflammatory cytokines and chemokines. IL‑17C action supports the integrity of the colon epithelium following infection induced damage but also contributes to psoriatic skin thickening and the progression of arthritis. IL‑17C is additionally up‑regulated in Th17 cell dependent autoimmunity. In this setting, it exacerbates disease severity by inducing Th17 cell production of IL‑17A, IL‑17F, IL‑22, CCR6, and CCL20.
• Source: E. coli
• Species: Human
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Human IL-17 RE Fc Chimera is immobilized at 0.25 μg/mL (100 μL/well), the concentration of Recombinant Human IL-17C that produces 50% of the optimal binding response is 1.5-7.5 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 19.8 (monomer) kDa
• Protein length: His19-Val197
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: IL17C interleukin 17C [ Homo sapiens ]
• Official Symbol: IL17C
• Synonyms: IL17C; interleukin 17C; interleukin-17C; CX2; IL 17C; IL 21; MGC126884; MGC138401; cytokine CX2; IL-21; IL-17C
• Gene ID: 27189
• mRNA Refseq: NM_013278
• Protein Refseq: NP_037410
• MIM: 604628
• UniProt ID: Q9P0M4
• Chromosome Location: 16q24
• Function: cytokine activity