Recombinant Human IL17RA protein, His-tagged

• Cat.No.: IL17RA-1741H
• Product Overview: Recombinant Human IL17RA aa. (Pro377~Phe534 (Accession # Q96F46)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Interleukin 17A (IL17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The transmembrane protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Pro377~Phe534 (Accession # Q96F46)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.4.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH 7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: IL17RA interleukin 17 receptor A [ Homo sapiens (human) ]
• Official Symbol: IL17RA
• Synonyms: IL17RA; interleukin 17 receptor A; CD217; IL17R; IMD51; CANDF5; CDw217; IL-17RA; hIL-17R; interleukin-17 receptor A; IL-17 receptor A
• Gene ID: 23765
• mRNA Refseq: NM_001289905.1
• Protein Refseq: NP_001276834.1
• UniProt ID: Q96F46

Reviews

01/23/2021

Effective in T-cell activation.

07/12/2019

Suitable for co-immunoprecipitation.

03/17/2017

High solubility.

Q&As

What experimental techniques are employed to investigate the interaction between IL17RA and various IL17 family members, and how is their binding specificity determined?

Surface plasmon resonance, co-immunoprecipitation, and mutagenesis studies reveal IL17RA's binding partners and affinity.

Can you discuss the experimental designs used to examine the crosstalk between IL17RA signaling and other immune pathways, such as TNF-α or IFN-纬 pathways?

Cross-pathway signaling assays, gene expression profiling, and functional rescue experiments unveil IL17RA's interaction networks.

What strategies are utilized to explore the contribution of IL17RA to tissue remodeling processes, such as angiogenesis and fibrosis?

Angiogenesis assays, fibroblast activation assays, and tissue remodeling models illuminate IL17RA's role in tissue repair.

How is the potential of IL17RA as a therapeutic target evaluated in experimental settings, and what challenges exist in translating these findings to clinical applications?

Animal models, pharmacological interventions, and toxicity assessments evaluate IL17RA-targeted therapies and translational challenges.

How do researchers study the involvement of IL17RA in autoimmune disorders through patient samples, and what insights have been gained?

Patient cohorts, clinical data analysis, and immune profiling provide insights into IL17RA's relevance in autoimmune diseases.

How do researchers study the role of IL17RA in the regulation of immune cell recruitment and infiltration in inflammatory tissues?

Flow cytometry, in vivo imaging, and chemotaxis assays characterize immune cell responses guided by IL17RA activation.

Can you elaborate on the methods used to dissect the intracellular signaling pathways triggered by IL17RA activation, especially in the context of autoimmune diseases?

Phosphorylation assays, gene expression analysis, and knockout models dissect IL17RA-mediated signaling cascades.

Could you explain the experimental approaches employed to assess the impact of IL17RA inhibition on chronic inflammatory diseases in preclinical models?

Disease models, antibody-based treatments, and inflammatory biomarker assessments elucidate IL17RA's therapeutic potential.

What molecular methods are employed to understand the genetic and epigenetic regulation of IL17RA expression in immune cells?

ChIP-sequencing, promoter analysis, and epigenetic modification studies uncover IL17RA gene regulation mechanisms.

What techniques are used to investigate the role of IL17RA in maintaining epithelial barrier integrity and preventing microbial invasion?

Epithelial barrier assays, microbiome analysis, and infection models decipher IL17RA's role in mucosal defense.