Recombinant Human IL1F6

• Cat.No.: IL1F6-317H
• Product Overview: Recombinant Human IL-1F6 produced in E.coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17.7 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Human interleukin family 1, member 6 (hIL-1F6 / FIL1-epsilon) is a member of the interleukin 1 cytokine family. IL-1F6 is expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines.IL-1F6 is the only IL-1 family member found to be expressed on T-cells. IL-1F6 is reported to function via receptor complex of IL-1Rrp2 and IL-1RAcP. Recombinant IL-1F6, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NFκB and MAPK in an IL-1Rrp2 dependent manner.
• Biological Activity: As measured by its binding ability in a functional ELISA, immobilized rHuIL-1F6 at 1 μg/ml (100 μl/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15~5 μg/ml.
• Purity: Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Lys-Ala-Leu.
• Endotoxin: Less than 0.1ng/µg (1 IEU/µg) determined by LAL test.
• Form: The protein was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4.
• Reconstitution: It is recommended to quick spin followed by reconstitution of rHuIL-1F6 in PBS to a concentration no less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Lyophilized rHuIL-1F5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-1F6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Gene Information

• Gene Name: IL1F6 interleukin 1 family, member 6 (epsilon) [ Homo sapiens ]
• Official Symbol: IL1F6
• Synonyms: IL1F6; interleukin 1 family, member 6 (epsilon); FIL1; FIL1E; IL-1F6; MGC129552; MGC129553; IL1(EPSIL- ON); FIL1(EPSILON); interleukin-1 family member 6 ; FIL1 epsilon ; IL-1 epsilon ; OTTHUMP00000203688 ; interleukin-1 epsilon ; IL-1F6 (FIL-1-epsilon) ; interleukin 1, epsilon ; Interleukin-1 epsilon
• Gene ID: 27179
• mRNA Refseq: NM_014440
• Protein Refseq: NP_055255
• MIM: 605509
• UniProt ID: Q9UHA7
• Chromosome Location: 2q12-q14.1
• Function: cytokine activity; interleukin-1 receptor binding