Recombinant Human IL1F6
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Cat.No. : | IL1F6-317H Optional Service: Optional requirements on this protein |
Product Overview : | Recombinant Human IL-1F6 produced in E.coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17.7 kDa. |
Description : | Human interleukin family 1, member 6 (hIL-1F6 / FIL1-epsilon) is a member of the interleukin 1 cytokine family. IL-1F6 is expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines.IL-1F6 is the only IL-1 family member found to be expressed on T-cells. IL-1F6 is reported to function via receptor complex of IL-1Rrp2 and IL-1RAcP. Recombinant IL-1F6, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NFκB and MAPK in an IL-1Rrp2 dependent manner. |
Biological Activity : | As measured by its binding ability in a functional ELISA, immobilized rHuIL-1F6 at 1 μg/ml (100 μl/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15~5 μg/ml. |
Purity : | Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
Amino-Acid Sequence : | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Lys-Ala-Leu. |
Endotoxin : | Less than 0.1ng/µg (1 IEU/µg) determined by LAL test. |
Form : | The protein was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4. |
Reconstitution : | It is recommended to quick spin followed by reconstitution of rHuIL-1F6 in PBS to a concentration no less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage : | Lyophilized rHuIL-1F5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-1F6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
Gene Name : | IL1F6 interleukin 1 family, member 6 (epsilon) [ Homo sapiens ] |
Official Symbol : | IL1F6 |
Synonyms : | IL1F6; interleukin 1 family, member 6 (epsilon); FIL1; FIL1E; IL-1F6; MGC129552; MGC129553; IL1(EPSIL- ON); FIL1(EPSILON); interleukin-1 family member 6 ; FIL1 epsilon ; IL-1 epsilon ; OTTHUMP00000203688 ; interleukin-1 epsilon ; IL-1F6 (FIL-1-epsilon) ; interleukin 1, epsilon ; Interleukin-1 epsilon |
Gene ID : | 27179 |
mRNA Refseq : | NM_014440 |
Protein Refseq : | NP_055255 |
MIM : | 605509 |
UniProt ID : | Q9UHA7 |
Chromosome Location : | 2q12-q14.1 |
Function : | cytokine activity; interleukin-1 receptor binding |
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◆ Lysates
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IL10IL10RAIL10RBIL11IL11RAIl11ra1IL12IL12AIL12BIL12RB1IL13IL13RA1IL13RA2IL15IL15RAIL16IL17AIL17BIl17cIL17DIl17fIL17RAIL17RDIL18IL18BPIL18R1IL18RAPIl19Il1aIl1bIL1F10IL1R1Il1r2IL1RAPIL1RAPL1IL1RAPL2IL1RL1IL1RL2Il1rnIL2Il20IL20RAIL21IL21RIL22IL22BPIL22RA1IL22RA2IL23AIL24Il25IL27IL27RAIL28AIl28bIL29IL2RAIL2RBIl2rgIL3IL31IL32Il33Il34IL3RAIL4IL4RIl4raIl5IL5RAIl6IL6RIl6raIL6STIl7IL7RIL8IL9IL36AIl12rb2IL17RBIL23RIL26IL31RAIL36BIL36GIL36RNIL37IL9RIL17RCIL17REIL28RAIL4I1IL20RBIL17RELIl11ra2IL27bIL7RAIl23a&Il12b
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