| Cat.No. : |
IL1F6-317H
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant Human IL-1F6 produced in E.coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17.7 kDa. |
| Description : |
Human interleukin family 1, member 6 (hIL-1F6 / FIL1-epsilon) is a member of the interleukin 1 cytokine family. IL-1F6 is expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines.IL-1F6 is the only IL-1 family member found to be expressed on T-cells. IL-1F6 is reported to function via receptor complex of IL-1Rrp2 and IL-1RAcP. Recombinant IL-1F6, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NFκB and MAPK in an IL-1Rrp2 dependent manner. |
| Biological Activity : |
As measured by its binding ability in a functional ELISA, immobilized rHuIL-1F6 at 1 μg/ml (100 μl/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15~5 μg/ml. |
| Purity : |
Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Amino-Acid Sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Lys-Ala-Leu. |
| Endotoxin : |
Less than 0.1ng/µg (1 IEU/µg) determined by LAL test. |
| Form : |
The protein was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4. |
| Reconstitution : |
It is recommended to quick spin followed by reconstitution of rHuIL-1F6 in PBS to a concentration no less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage : |
Lyophilized rHuIL-1F5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-1F6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
